The Northern Blot test is used to identify tissue specific protein expression by detecting mRNA in a sample.
- RNA samples are separated via. gel electrophoresis
- RNA samples that have now been separated by size are transferred to a nylon membrane
- Once RNA is transferred it is fixed to the nylon membrane using heat or UV light.
- Once RNA is fixed to the nylon sheet the hybridization probes are applied to the membrane.
- Prior to imaging, the membrane is washed to remove any additional probe.
- Hybrid signals are then detected by X-ray film.
This can help to determine if gene expression is tissue specific or to determine when certain genes are expressed.
Wikipedia. Northern Blot. Last modified: Sep 19 2012. http://en.wikipedia.org/wiki/Northern_blot