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High-throughput sequencing, aka next-generation sequencing, is based on massive parallel sequencing which allows the increase of the capacity and effectivity of sequenators while reducing the sequencing price. The original method of the next-generation sequencing is Massively Parallel Signature Sequencing - MPSS, which, based on the number of mRNA molecules produced by each gene, and it analyzes their expression rate. MPSS was soon overcome by other, more effectid methods, such as pyrosequencing (using bioluminiscence), Illumina (Solexa) sequencing or SOLiD sequencing.

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