DNAseq: Maxam - Gilbert[]
This method of sequencing is also known as chemical sequencing and utilizes radioactive isotopes. One will have purified double stranded DNA, which will then be labeled at the 5' end of the DNA with a radioactive isotope. Chemicals are used to essentially break the DNA in small increments and the products of the reaction are run on a gel to ultimately determine the sequence through mobility of the generated fragments. Gels are visualized using x-ray film.
This method is not used as frequently with the advent of other sequencing techniques. (such as the Sanger method.)
Maxam-Gilbert Sequencing. From Matthews and van Holde: Biochemistry, 2nd edition. The Benjamin-Cummings Publishing Company. http://www.nd.edu/~aseriann/maxam.html