The Ion Torrent technology works by measuring the change in pH after each base is translated. The product is made from three layers: a layer of micro-machined wells, an ion-sensitive layer, and a proprietary ion sensor layer. Each well holds a DNA template. When a nucleotide is added to a template, a hydron is released into the solution which is charged to a specific pH. The change in pH is measured by the ion sensor and translated into digital information. The order of the bases and base letters can be viewed on a screen.
- Microbial Sequencing
- Mitochondrial Sequencing
- "16 samples per run with barcoding"
- Amplicon Sequencing
- This uses PCR-based amplification method. The PCR-based method amplifies regions of the genome by using specific enzymes.
- Good for low-input DNA samples
- Targeted Resequencing
- Uses In-Solution Hybridization-Based Pullout Method
- Good for viewing exons or large sets of genes
- Can be used for "structural variation detection"
- RNA Sequencing
- Validation and Library Assessment
- Exome Sequencing
- Human-Scale Genome Sequencing
- Chromatin Immunoprecipitation Sequencing
- Whole-Transcriptome Sequencing
All textual information was acquired from the Life Technologies Website in the Ion Torrent Section: Life Technologies