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OverviewEdit

The Ion Torrent technology works by measuring the change in pH after each base is translated. The product is made from three layers: a layer of micro-machined wells, an ion-sensitive layer, and a proprietary ion sensor layer. Each well holds a DNA template. When a nucleotide is added to a template, a hydron is released into the solution which is charged to a specific pH. The change in pH is measured by the ion sensor and translated into digital information. The order of the bases and base letters can be viewed on a screen.

UsesEdit

  • Microbial Sequencing
  • Mitochondrial Sequencing
    • "16 samples per run with barcoding"
  • Amplicon Sequencing
    • This uses PCR-based amplification method. The PCR-based method amplifies regions of the genome by using specific enzymes.
    • Good for low-input DNA samples
  • Targeted Resequencing
    • Uses In-Solution Hybridization-Based Pullout Method
    • Good for viewing exons or large sets of genes
    • Can be used for "structural variation detection"
  • RNA Sequencing
  • Validation and Library Assessment
  • Exome Sequencing
  • Human-Scale Genome Sequencing
  • Chromatin Immunoprecipitation Sequencing
  • Whole-Transcriptome Sequencing

VideosEdit

http://www.youtube.com/watch?v=yVf2295JqUg&feature=related

http://www.youtube.com/watch?v=jv-JHafk4UA&feature=relmfu

ReferencesEdit

All textual information was acquired from the Life Technologies Website in the Ion Torrent Section: Life Technologies

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