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Conditional Mutant

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Conditional mutant of a gene only expresses the gene product only in the presence or absence of a repressor or inducer. This system is used the analyze the function of important or essential genes or mutations that could otherwise be deleterious. There are several methods of making conditional mutants including the tetracycline inducible/ repressible system, Destabilization Domains (PMID:16959577), IPTG induction of lacZ operon, temperature sensitivity.

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